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  Sequencing

  From the sample to the DNA sequence

Most of the time, the DNA/RNA sequencing process is the first step of the services Genostar provides. From your biological sample, we extract the DNA/RNA, check its quality and potential contaminations, sequence the DNA/RNA and assemble the sequences. 

 

 

All major sequencing technologies are available, from Sanger sequencing to NGS (Next-Generation Sequencing) and TGS (Third-Generation Sequencing) technologies. Depending on your research project, our bioanalyst may recommend one of these technology, or a combination of 2 or 3 of them. 

 

DNA and RNA sequencing technologies available:

  • Sanger sequencing
  • 454 (Roche)
  • HiSeq and MiSeq (Illumina)
  • PacBio RS (Pacific Bioscience)
We can perform Multiple Locus Genome Sequencing (MLST), Whole Genome Sequencing (WGS), and genome recircularisation.

 

Overall process flow:

  • DNA extraction
  • DNA / RNA depletion if necessary
  • Contamination control before sequencing (16S RNA and/or ITS sequencing)
  • Filtering / trimming
  • Sequencing
  • Assembly of the reads into contigs and scaffolds
  • Generation of a flat file 
  • Personalised report
  • Data backupp

 

At the end of the process, if you wish a complete annotated genome (GenBank or EMBL file), you should ask for the annotation of your newly sequenced genome.